منابع مشابه
The Determination of Blood Urea
Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...
متن کاملThe Determination of Blood Urea
Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...
متن کاملA Coulometric Determination of Urea Nitrogen in Blood and Urine.
Since the ammonia undergoes a 3-electron change, 1 mole is equal to 3 equivalents of the generated titrant. Recently we (lescril)ed an improved cud-point (letection for this system and applied it to titration of as little as 1.4 g. ammonia (2). This method has been extended to titration of ammonia formed by the action of urease on urea in blood and urine. In urine samples, ammonia may be titrat...
متن کاملA New Method for the Determination of Urea in Blood
In a recent number of this Journal,’ a new method for the estimat,ion of urea in urine was described. This method consists in the conversion of the urea into ammonium carbonate by means of the urease of the soy bean, and an estimation of the alkalinity before and after the conversion by means of standard acid and methyl orange. The procedure is not directly applicable to the est,imation of urea...
متن کاملThe Direct Colorimetric Determination of Urea in Blood and Urine
Methods for the determination of urea in biological fluids have passed through an interesting cycle (cf. Peters and Van Slyke (3) pp. 539-544). The earliest procedures were strictly chemical ones, involving precipitation of the urea as an insoluble mercury complex or decomposition of the urea by means of heat or hypobromite. The use of the highly specific enzyme urease occurring in the jack and...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1928
ISSN: 0306-3283
DOI: 10.1042/bj0220545